Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Auburn University at Montgomery |
| Observations | Climbed into net. Managed to contain and place it in a jar. |
| Putative identification | Arthropoda Insecta Hemiptera Lygaeidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Taq polymerase = GO Taq MM 55 degrees Celsius for both PCR reactions. (Annealing temperature) The gel electrophoresis did not show a band for either the Arthropod or Wolbachia. It could be because I put too much of the sample in the tube, or other errors. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
ET1
Harmonia Axyridis
SF 1
Coccinellidae