DEM-WAO Coleoptera

April 13, 2026

Sample information
Picture
Entry by: William O
Location
Collection date 02/09/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

According to prior results, Coleoptera prevalence was approximately 23%
Wolbachia 16S sequence
Arthropod COI sequence
GGTTTAGTAGGAACTTCTTTAAGAATATTAATTCGAACAGAGCTAGGTAG CCcctgatCATTAATTGGAAATGACCAAATCTATAATGTAATTGTTACat cCCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATTATAATTGGA GGATTTGGAAATTGATTAGTACCACTAATAATTAGGGCCCCTGATATAGC ATTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCTTCACTAT TTTTACTAATTATAAGAAGAATTGTTGAAAATGGAGCAGGAACAGGATGA ACTGTATACCCCCCTTTATCATCTAACTTAGCTCATGAAGGATCTTCAAT TGATTTAGCAATTTTTAGACTACATTTAGCAGGAATTTCTTCTATTCTTG GAGCAATTAATTTTATTACAACAGTAATTAATATACGACCAATTGGTATA ACCCTAGATCAAATACCGTTATTTGTATGGGCTGTAACAATTACAGCTAT TTTATTACTATTATCTTTACCAGTATTAGCAGGAGCTATCACAATATTAT TAACTGACCGAAATTTAAATACTTCTTTTTTTGATCCTGCAGGAGGAGGT GATCCTATCCTTTATCAACACCTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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