BRO-ACO Diptra

April 14, 2026

Sample information
Picture
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time of Collection: 10:00-10:30 a.m.
  • Location of Collection: Science Center, Armstrong Campus
  • Description of Environment: Outside in bushes, dirt, and trees
  • Method of Collecting: Digging
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my insect was not infected with Wolbachia.
Wolbachia 16S sequence
GTGGCGTGCTGATCCACGATTACTAGCGatcccttcttcgtgCACTCGAGTTGCAGAGTACAATCCGAACTGAgaTggcTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATAGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GTAGGAACTTCACTTAGTATACTTATTCGAGCAGAACTTAATCAACCCGG ATCTCTTATTGGAGATGATCAAATCTATAATGTAATTGTAACAGCACACG CCTTCATTATAATTTTCTTTATAGTAATACCCATCCTAATTGGTGGATTT GGGAATTGACTAGTTCCTCTTATATTAGGAGCGCCAGATATAGCTTTTCC ACGAATAAATAATATAAGATTCTGATTATTACCTCCTTCTTTGACCCTGC TGTTGGCTAGAAGAATAGTAGAAAGCGGAGCTGGTACTGGATGAACAGTT TATCCCCCACTTGCAAGAAGCATTGCACATGCTGGAGCATCTGTAGACCT TGCTATTTTTTCTCTACATCTTGCAGGAGTGTCTTCAATTCTAGGAGCAG TAAACTTTATCTCAACAATTATTAATATAAAACCAATCAGCATAAAACCT GAACGTATTCCTTTATTTGTATGAGCTGTTGGAATTACTGCTTTACTTCT TCTTTTATCCTTGCCGGTCCTTGCAGGAGCAATTACTATACTTCTTACTG ATCGAAATTTAAATACCTCCTTCTTTGACCCTGCTGGAGGAGGAGATCCT ATTCTTTACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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