BRO-MKJ Hymenoptera

April 14, 2026

Sample information
Picture
Entry by: MaKenzie J
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date of Collection: 01/27/2026​
  • Time of Collection: 10:45 AM​
  • Location of Collection: Savannah, Georgia (outside ScienceBuilding)​
  • Description of Environment: under leaves, dug in the dirt​
  • Method of Collection: scooping dirt on a tray and sifting through tofind insects​
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Through the testing we completed in class as well as my own personal research I am very confident on my results.
Wolbachia 16S sequence
Arthropod COI sequence
CTTGATCGGGgatgGTAGGAACTAATTTGAGAATTTTAATTCGAACAGAG TTAGGTCacccttgatgtTTAATTGGAGATGATCAGATTTATAATGTTAT TGTTACTGCTCACGCTTTTATTATAATTTTTTTTATGGTAATGCCAATTA TAATTGGAGGGTTTGGTAATTGATTAGTTCCTTTAATATTGAGAGCTCCA GATATAGCTTTCCCACGAATGAATAATATAAGATTTTGGTTATTACCTCC ATCTCTGATTTTATTATTATCAGGGGGCCTAGTAGATAGAGGGGCTGGGA CAGGTTGGACAGTGTATCCTCCTTTATCATCAGTGATTTCTCATGGAGGG GCTTCAGTGGATATAACTATTTTTTCTTTACATTTGGCAGGGGTTTCTTC TATTTTGGGAGCCATTAATTTTATTACTACTGTGATTAATATACGTCCAG AAGGTTTAAAGCTTGATCGTGTTCCTTTATTTGTTTGATCAGTAGCTATT ACTGCTTTATTATTATTGCTTTCTTTACCGGTTTTAGCAGGAGCTATTAC GATATTATTAACTGATCGAAATTTAAATACATCTTTTTTTGATCCAGCCG GAGGCGGAGATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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