BRO-DJN Hemiptera

April 14, 2026

Sample information
Picture
Entry by: Darius N
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

“DNA extraction kit of in-house reagents was used.”

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Very high

Co1 Sequence:

AGTTGGGTCATCCTTAAGATGAATTATTCGAATTGAACTGGGCcaccctt

gttgatTTATTGGAGATGATCAAATTTATAATGTAATTGTAaCTGCTCAT

GCATTTATTATAATTTTCTTTATAGTTATGCCAATTATAATTGGAGGTTT

TGGTAATTGGCTAGTACCTTTAATAATTGGTGCACCAGATATAGCATTCC

CCCGAATAAATAATATAAGTTTTTGGTTATTACCCCCATCATTAACCCTA

CTATTAAGAAGTAGCATGGTTGAAATAGGGGCAGGAACAGGTTGAACTGT

TTATCCTCCATTATCAAGCAATATTTCACATAGAGGGGCTTCAGTAGACT

TAGCTATTTTTTCCCTCCATTTAGCAGGAGTTTCATCTATTTTAGGAGCA

ATTAATTTTATCTCAACTATTATAAATATACGCCCAGCAGGTATAATTCC

TGAGCGAACTCCTCTCTTTGTATGATCAGTGGGAATTACTGCTCTTCTAT

TACTTTTATCCTTACCTGTTTTAGCAGGGGCTATCACTATATTATTAACT

GATCGTAATTTTAATACTTCCTTTTTTGACCCCACTGGTGGAGGAGACCC

TATCCTTTATCAACACTTATTTTGATTTTTTGGTCA
Wolbachia 16S sequence
GTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCAt gctctCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATT AGCTTAGCCTTGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGT GTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTT CCTCCAGTTTATCACTAGCAGTTTCCTTAAAGTCCCCAGCATTACCTGAT GGTAACTAAGGATGAGGGTTACGCTCGTTGCGGGACTTAACCCAACATCT CACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAATCCGGCCG AACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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