MAN-MRC Hymenoptera

April 14, 2026

Sample information
Picture
Entry by: Makayla C
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time Captured: 3-3:10pm

Habitat: Flower Bush

Method of Collection: Big Jar
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Bands were clear, controls came back as expected, good quality samples used
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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