Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/28/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I thought my sample showed the wolbachia gene in the gel electrophoresis, but sequencing showed no wolbachia present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATTGGTTCATCTATAAGAATAATCATCCGATTAGAACTAGGATCTTGtta tTCTCTAATTAATAATGATCAAATCTACAACTCCCTAGTTACTAGACACG CTTTTATTATAATTTTTTTTATAGTTATGCCCTTCATAATTGGGGGATTT GGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCTGATATGGCCTATCC TCGAATAAATAATATAAGATTTTGACTTCTACCACCTTCTCTAACTCTTT TACTTATCAGAAGATTTATTAATAGAGGAGTGGGGACAGGATGAACTATC TATCCACCACTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGACCT ATCTATTTTTTCCTTACATATCGCCGGAATATCATCAATCTTAGGGGCTA TTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTACTATA GATAAAATCCCCCTACTAGTCTGATCTATCCTTATTACAGCCATTCTTCT TTTACTTTCCCTCCCAGTTCTTGCAGGAGCTATTACAATATTACTAACTG ACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGGGAGGGGGAGATCCC ATTCTATatcAACATTTATTCTGATTTTTTGGTCACC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
DEM-AES – Hymenoptera
DEM-ORK Order Hymenoptera
(no title)
DEM-OKA Orthoptera
DEM-MGC Coleoptera