Common wood lice

July 16, 2021

Sample information
Picture
Photos by: Nora G
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Arthropod was found in an shaded field next to a river. The It has an hard, shiny exoskeleton, and a black stripe down the dorsal side of its back.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Philosciidae Philoscia Philoscia muscorum

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I used the abdomen of this bug, because it had a hard shell. This bug is loacated in the sixth well.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Philoscia muscorum was found to be postive for Wolbachia.
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