weevil

July 16, 2021

Sample information
Picture
Photos by: JunoY
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Caught on notebook on grass. bark like shell. very small.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Curculionidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The entire bug was mashed.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

It was next to the positive well. It is extremely hard to see. The band is so faint the camera could not see it and can only see it at an angle.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Curculionidae was found to be postive for Wolbachia.
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