Sample information |
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Picture |
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Location | |
Collection date | 07/07/2021 |
Captive / Cultivated? | Wild-caught |
Group | Norwich Free Academy |
Observations | Caught on notebook on grass. bark like shell. very small. |
Putative identification | Arthropoda Hexapoda Insecta Coleoptera Curculionidae |
Methods |
|
Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Fast Blast |
Gel images |
|
Protocol notes | The entire bug was mashed. |
Results |
|
Wolbachia presence | Yes |
Confidence level | Low |
Explanation of confidence level | It was next to the positive well. It is extremely hard to see. The band is so faint the camera could not see it and can only see it at an angle. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Curculionidae was found to be postive for Wolbachia. |