CHI-KFB Diptera

April 16, 2026

Sample information
Picture
Entry by: Kaleigh B
Location
Collection date 01/29/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Bug was given to me by lab instructor.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All control results show as expected
Wolbachia 16S sequence
Arthropod COI sequence
TTGGAGCTTGAGCAGGAATAGTAGGAACTTCTCTTAGTATTTTAATTCGA GCAGAATTAGGACATccatgtGCTTTAATTGGAGATGATCAAATTTATAA TGTAATTGTTACAGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATAC CTATTATAATTGGAGGGTTTGGAAATTGATTAGTTCCTCTAATATTAGGA GCTCCTGATATAGCTTTCCCTCGAATGAATAATATAAGTTTTTGATTACT ACCTCCTTCTCTTACCTTACTTTTAGTAAGCAGTATAGTAGAAAACGGAG CTGGTACAGGATGAACAGTTTACCCACCGCTATCTTCTGGAATTGCTCAT GGTGGAGCATCAGTAGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAGT TTCTTCTATTTTAGGAGCTGTAAATTTTATTACAACAGTAATTAATATAC GATCTACAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCTGTT GTAATTACAGCTTTATTATTACTTTTATCTTTACCAGTATTAGCTGGAGC TATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGATC CTGCTGGAGGAGGGGATCCTATTTTATATCAACATTTATTTTGATTTTTT GGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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