BRI-MAO COLEOPTERA

April 16, 2026

Sample information
Picture
Entry by: Maya O
Location
Collection date 01/22/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The insect has mandibles, two sets of wings and 6 legs.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high because the average presence of Wolbachia for my specimen is low. On top of that, my results show my specimen is negative for the Wolbachia gene.
Wolbachia 16S sequence
n/a
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTCCTATTTggtaGATGAGCAGGaataGTAGGAACCTCCCTTAGACTACTAATTCGaGCAGAACTCGGAAATCCTGGATCTCTGATTGGAGACGACCAAATTTACAATGTTATTGTTACTGCACATGCTTTCATCATAATTTTTTTCATGGTAATACCAATTATAATTGGTGGATTCGGAAACTGGCTTGTCCCTCTAATACTTGGGGCCCCTGACATAGCTTTCCCCCGAATAAATAACATAAGATTTTGGCTTCTTCCTCCTTCTTTAACTTTACTTTTAATGAGAAGATTAGTAGAAAGAGGAGCTGGAACTGGGTGAACAGTATACCCCCCTTTATCTAGAAACATCGCTCACAGAGGGGCTTCTGTAGATCTAGCTATTTTTAGTTTACACCTAGCTGGAATTTCATCTATTCTTGGAGCTGTAAATTTCATTACTACAGTTATTAACATACGtTCTACAGGCATAACATTTGACCGAATACCATTATTTGTATGATCagtagctTTAACaacTCTTTTACTACTTCTGTCCCTGCCTGTCCTAGCTGGCGCAATCACGATACTTTTAACTGATCGAAATATTAATACCTCATTCTTTGACCCAGCGGGTGGTGGAGACCCAATTTTATACCAACACTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post