Wood Roach

July 16, 2021

Sample information
Picture
Photos by: Kevin W.
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Segmented, oval body, long antennae, 6 legs, brown in color, collected in shaded area in leaf litter.
Putative identification Arthropoda Insecta Blattodea Cryptocercidae Cryptocercus

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Used abdomen of wood roach. In 3rd well.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Band was blurry, positive control N/A, slightly lower than SS
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cryptocercus was found to be postive for Wolbachia.
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