Sample information
Picture	Entry by: Omar M.
Location
Collection date	09/03/2025
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	collected by Fall 2025 students
Putative identification	Arthropoda Insecta Hymenoptera Vespidae Eumenes Eumenes fraternus
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	Confirmed BLAST results
Wolbachia 16S sequence	Download FASTA
Arthropod COI sequence	Download FASTA
Summary	The Eumenes fraternus was found to be postive for Wolbachia.