BASD-flying ant

May 4, 2026

Sample information
Picture
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

I got my insect off a car in the parking lot at school. The weather was standard late summer weather in Pennsylvania, around 65 degrees. My identification of the insect isn’t certain but believed to be a flying ant due to research.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain UView
Gel images
Protocol notes

I dissected the abdomen, and left on the exoskeleton. I made sure to crush the insect very well.
1-ladder

2-flying ant(this sample)

3-insect 2(gnat)

4-positive fruit fly

5-negative fruit fly

6-positive DNA

7-negative with water

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All the controls came out normal, and the one of the unknowns came out positive and the other didn’t. The bands were where they were supposed to be. I’m confident this insect does have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
Report Inappropriate Post