Sample information
Picture	Entry by: Ava H
Location
Collection date	04/21/2026
Captive / Cultivated?	Wild-caught
Group	Winterset High School
Observations	Ticks Woody areas
Putative identification	Arthropoda Arachnida Ixodida
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	The line was faint
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Ixodida was found to be postive for Wolbachia.