House centipede Wolbachia

May 28, 2026

Sample information
Picture
Entry by: Abdelrahman Zannoun
Location
Collection date 03/13/2026
Captive / Cultivated? Wild-caught
Group Lafayette High School
Observations

Appeared larger with more limbs before being caught and stored in freezer. Discoloring occurred as well as it transitioned from a pale yellow to a darker blue as well.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system BioRad
Buffer 1X TAE
DNA stain Cyber Green
Gel images
Protocol notes

Agarose gel was used to run electrophoresis on samples after dna extraction.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I can see that the band is clearly not present, however the bands are somewhat blurry and not consistent.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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