Spider

June 2, 2026

Sample information
Picture
Entry by: Iris A.
Location
Collection date 04/20/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Found on a rock near a stream.
Putative identification Arthropoda Arachnida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA Extraction: Abdomen was dissected and crushed using a pestle.
Analysis: The PCR likely was unsuccessful, as all of the gel lanes except the ladder do not contain any bands. The positive control failed.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The images of the gel are unclear/blurry and the PCR likely was not successful.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post