Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 04/25/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | |
| Putative identification | Arthropoda Insecta Coleoptera Curculionidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR-SAFE |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The positive control did not show a band as expected therefore it is a low confidence level as to whether this arthropod has Wolbachia or not |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CAGGAATAGTGAAGTACATCCCTAAGCCTACTAATTCGATCTGAACTAGGAAATCCCGGATCATTAATTGGTAACGATCAAATTTATAATGTTATTGTTACAGCACATGCTTTTATTATAATTTTTTTCATAGTTATACCTATTTTAATTGGGGGTTTTGGAAATTGATTAGTCCCACTAATATTAGGTGCACCTGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCATCAATTTCGTTATTAATTATAAGAAGAGTTGTAGAAAATGGTGCAGGAACAGGATGAACTGTGTATCCCCCACTTTCATCTAATATTGCCCATAGAGGATCCTCTGTAGATTTAGCTATTTTTAGTCTTCATTTAGCTGGGATTTCTTCTATTCTAGGGGCAGTAAATTTTATTACTACAATTATTAATATACGTCCAGTAGAAATAACATTTGATCGATTACCACTTTTCGTATGAGCTGTAATAATTACTGCAATTTTATTGCTTCTTTCTCTACCAGTATTAGCAGGTGCTATTACTATATTACTTACCGATCGTAATTTAAATACCTCGTTTTTTGACCCAGCTGGTGGGGGAGATCCGATTCTTTACCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Curculionidae was found to be negative for Wolbachia. |
Eudioctria albius (Robber Fly, Wolbachia -)
The fANTastical four
Pepsinae (Spider Wasp, Wolbachia +)
Camponotus nearcticus (Wolbachia +)
Colonus sylvanus (Sylvan Jumping Spider, Wolbachia +)