Water Strider

June 10, 2026

Sample information
Picture
Entry by: Iliaus G M
Location
Collection date
Captive / Cultivated? Captive / Cultivated
Group Thomas Jefferson High School for Science and Technology
Observations

Caught on the water while mating with another water strider
Putative identification Arthropoda Insecta Hemiptera Gerridae Aquarius Aquarius remigis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Arthropod was cut up and and inside “goop” was used.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

No electrophoresis for Wolbachia sequencing showed bands strong enough for a positive, including the positive control.  This indicates some error occurred during Wolbachia sequencing and it is unknown whether the Water Strider had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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