Black-bordered Lemon Moth (Marimatha Nigrofimbria)

October 6, 2021

Sample information
Picture
Photos by: Sara P., Solomon D., Sabina T., and Sofia P.
Location
Collection date 08/22/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Weather: 29℃

Habitat: Terrestrial Plains

Elevation: 191.26 m
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Noctuidae Marimatha Marimatha nigrofimbria

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The samples have been tested by students and they have also been tested and sequenced from Wolbachia Project responsible personnel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Marimatha nigrofimbria was found to be negative for Wolbachia.
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