Honey Bee- Hymenopterans

October 6, 2021

Sample information
Picture
Photos by: Amna A.
Location
Collection date 08/23/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
ATCAATGAGACTTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAACAATGATCAAATTTATAATACAATTG TTACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTGGAGGATTTGGAAATTGGCTTATTCCT TTAATACTAGGATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTTTGATTACTTCCTCCCTCATTATTTAT ACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATATCCACCATTATCAGCATATTTATATC ATTCTTCACCTTCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTTCCTCAATTATAGGATCATTAAACTTA ATAGTTACAATTATAATAATAAAAAATTTTTCTATAAATTATGACCAAATTTCATTATTTCCATGATCAGTTTTTATTAC AGCAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACTATTATTTGATCGAAATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hexapoda was found to be negative for Wolbachia.
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