Hapithus Agitator

September 10, 2026

Sample information
Picture
Entry by: Kaleigh B
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Observed arthropod on green shrubbery. Long hind legs with a thick round torso with 2 yellow stripes. 6 total legs and 2 long antennas.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR-SAFE
Gel images
Protocol notes

Gel electrophoresis system: Standard Electrophoresis. Buffer 1xTAE. DNA stain: SYBR Safe. Lane 1: DNA Standard Ladder Lane 2: JLAH01 (CO1+Wol) Lane 3: JLAH02 (CO1 +Wol) Lane 4: JLAH03 (Positive Control) Lane 5: JLAH04 (Negative Control) Lane 6: JLAH05 (Positive DNA Control) Lane 7 Water control Lane 8:Empty Lane 9: DNA Standard Ladder Lane 10: PFKB01 (CO1 +Wol) Lane 11: PFKB02 (CO1 +Wol) Lane 12: PFKB03 (Positive Control) Lane 13: PFKB04 (Negative Control) Lane14:PFBK05 (Positive DNA Control) Lane 15: Water control Lane 16: Empty
My lab partner and I were the bottom portion of gel.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls worked because the bands in the gel electrophoresis were present to ensure accuracy.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
Report Inappropriate Post