September 10, 2026

Sample information
Picture
Entry by: Ellena S
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Small, wedge-shaped insect with a blue-green gradient.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

First Lane: DNA ladder Standard

Second lane: Leaf hopper

Third lane: Termite (Wolbochia negative)

Fourth lane: Positive Control

Fifth lane: Negative Control

Sixth lane: DNA Positive Control

Seventh lane: Water

Eighth lane: Empty

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am confident in the results because of the comparison using positive and negative controls.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNNGNANTTGATCNGGACTAGTAGGAATAATAATAAGAATAATTATTCGAATAGAACTATCTCAACCAGGATCAATAATCAAAAATGACCAAATTTATAACACAATTGTAACTTCACATGCATTTGTAATAATCTTTTTTATAGTTATACCAATTATAATTGGTGGTTTTGGAAACTGGCTTGTTCCTCTTATAATTGGAGCACCAGATATAGCTTTCCCACGAATAAACAATATAAGTTTTTGACTTTTACCAATATCAATTACATTATTAATTGCAAGATCAACAGCAGCTTCAGGAACAGGAACAGGATGAACTGTATACCCACCTCTTTCGGCTCAAGAAGCACATTCAGGACCATCAGTAGACTTAACAATTTTTTCATTACACATTGCAGGTATTAGATCAATTATAGGAGCAATTAATTTTATTTCAACCATTATAAATATACGAATCAAAGGAATAACAATAGAAAAAACCCCCTTATTTTGTTGATCAGTACTAATTACAGCAATNCCTTTTACTAGTTTCTTTANCAGTACTANCCGGAGCANCNNNANATTNNNNNNNANNGAAANNNANTANNNNTTTTNNATCNTCNGNNNGNGGNNGANNCNNNTNNNGNANCACNNNCTNNNTNNNGNNTNNNNNNNNNNNNNNNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post