JWO 08 – Cabbage White Butterfly

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Large white wings, long antennae

On flower in HACC Pollinator Garden
Putative identification Arthropoda Insecta Lepidoptera Pieridae Pieris Pieris rapae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 8 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Bright CO1 band, no Wolbachia band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pieris rapae was found to be negative for Wolbachia.
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