JWO 10 – Faint Spotted Angle Moth

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.89
Collection date 09/03/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Large grey wings relative to body, long antennae.

Flying in woods near HACC parking lot.
Putative identification Arthropoda Insecta Lepidoptera Geometridae Digrammia Digrammia ocellinata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 10 on gel image.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Primer Dimers shown, no bands.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Digrammia ocellinata was found to be negative for Wolbachia.
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