Boxelder Bug (Hemiptera)

September 18, 2026

Sample information
Picture
Entry by: Ana Y
Location
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

The insect has a red body, black wings, long antenna’s.
Putative identification Arthropoda Insecta Hemiptera Rhopalidae Boisea Boisea trivittata

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

While Wolbachia is an incredible widespread in insects, the boxelder bug is expected to be negative for live infections due to their unique evolutionary and biological traits.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Boisea trivittata was found to be negative for Wolbachia.
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