Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 38.97, -77.08 |
| Collection date | 09/03/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Found under a desk indoors, captured in plastic container, put in freezer, covered in isopropanol Season: Late Summer Temperature: 70 F Tannish brown, no wings present, 6 legs, separated eyes, external mandible for chewing, enlarged hind legs for jumping, 2 elbowed antennae, 2 body regions, laterally compressed body |
| Putative identification | Arthropoda Insecta Orthoptera Rhaphidophoridae Diestrammena Diestrammena asynamora |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA Extraction During first centrifuge step, centrifuge was set to 10,000 rpm instead of 8,000 rpm. Corrected quickly. PCR Spilled a small amount of water on the table. Gel Electrophoresis D3 sample (not this one) had issues with DNA extraction and thus did not appear on the gel. This issue does not appear to have affected any other samples. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The protocol for this sample went on without any visible mistakes — we have a high confidence level in our gel results because positive and negative Wolbachia and Arthropod controls were included and the positive Arthropod and negative Wolbachia controls showed similar results in the gel |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Diestrammena asynamora was found to be negative for Wolbachia. |
CON-JRR Neuroptera
CON-RXB Hymenoptera
CONMED Diptera
CON-MAR Hemiptera
CON-CBS Blattodea