Sample information |
|
| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen/cut:partial |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Very confident as the control and insect bands came back as expected when doing the gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATAGTTGGAACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCA TCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAG TTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGA ATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGG TACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTC TTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATT ACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGC CGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCNNNGGGGGANACCCTATTTT ATACCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Insecta was found to be negative for Wolbachia. |
DEC-JAM Order Hemiptera
DECANA Hemiptera
(no title)
DECKNM Blattodea
DEM-RPW Odonata