DEMAACT – Orthoptera

November 2, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time Of Collection: 1200 – 1230
  • Location Of Collection: Georgia Southern University Armstrong Campus.
  • Description Of The Environment Of Collection Site: First Location,  Tallgrass;  Second Location,  Under Mulch.
  • Methods Of Collection:  Sweep Net, Hands.
Putative identification Arthropoda Insecta Orthoptera Tettigoniidae Conocephalus

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The prediction of the Orthoptera subject was negative based on a reference of data from 2016, and after the extraction of DNA, PCR and gel electrophoresis occurred it was shown that the results were negative as well.
Wolbachia 16S sequence
Arthropod COI sequence
CTCAATAAGATTATTAATTCGAGCTGAGCTAGGACAACCAG GTTTTCTTATTGGAGATGATCAAATTTATAACGTTATTGTAACGGCACATGCCTTTGTTATAATTTTTTTTATAGTTATA CCAATTATAATCGGAGGATTCGGAAATTGGTTAGTTCCTTTAATATTGGGGGCTCCAGATATAGCTTTCCCACGAATAAA CAATATAAGCTTTTGACTTTTACCCCCATCCTTGACCTTACTTCTAGCCAGAAGTCTTGTCGAAAACGGAGCAGGTACTG GTTGAACAGTTTATCCACCACTTTCTGCTAATATTGCGCATGCAGGAGCCTCAGTTGATCTAGCTATCTTCTCATTACAT TTAGCAGGTATTTCCTCAATTTTAGGGGCTGTAAATTTTATTACTACAACAATTAATATACGAGCCCCTGGAATATCCCT AGATCAAACCCCCTTATTTGTATGAGCAGTAGCTATCACAGCACTACTTTNATTATTATCTTTACCAGTATTAGCAGGAG CTATTACTATATTATTAACCGATCGAAATTTNAATACATCCTTCTTTGACCCTGCTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Conocephalus was found to be negative for Wolbachia.
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