Chi-JDB Hemiptera

November 3, 2026

Sample information
Picture
Entry by: Jayda Brooks
Location
Approximate location: 31.98, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught with gloved hands on a small landscape near the road in front of the UPD station.It was September 1, 2026. The time was around 10:21 a.m. The area already had many mosquitoes, and damp grass. For temperature it was hummid.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My positive, negative, NTC controls, as well as my partner’s DNA, performed correctly.
Wolbachia 16S sequence
GTGTACAGacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCATTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATCATCCCCATCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAGCATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACAC=CTGTGTAAAATCCGGCCGAACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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