MAT-JMW Hemiptera

November 3, 2026

Sample information
Picture
Entry by: Jenni W
Location
Approximate location: 31.97, -81.16
Collection date 09/08/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

2 pairs of wings. top wing pair are hard and half sized. Mouth parts are tube like and piercing sucking.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel electrophorsesis showed as expected results for control groups. Therefore supports lack of wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
AGTAGGAACAGCTATAAGATGAATTATTCGAATTGAACTTGGACAACCG GGAATATTTATTGGGGATGACCAAATCTATAATGTCATTGTAACAGCCCATGCATTCGTAATAATTTTTTTCATAGTAAT ACCAATCATAATTGGGGGTTTTGGAAACTGACTAGTACCCTTAATAATCGGAGCCCCTGATATAGCTTTCCCTCGAATAA ATAACATAAGATTTTGACTATTACCCCCTTCATTAACCTTACTACTCGTAAGCAGAATAGTAGAAATAGGAGCAGGAACT GGATGAACAGTTTATCCTCCATTATCCAGTAACCTCTCCCACAGAGGAGCATCCGTTGATTTAGCAATTTTTTCCCTACA TTTAGCAGGTGTATCCTCAATTCTCGGAGCTGTAAATTTTATCTCAACTATCATCAATATACGCCCTGCAGGAATAACAC CTGAACGAACACCTTTATTCGTTTGATCAGTAGGAATCACTGCACTACTATTATTACTATCATTACCTGTACTAGCAGGG GCTATTACTATATTATTAACAGATCGAAACTTTAATACCTCTTTCTTTGACCCCTCAGGTGGAGGAGACCCAATCCTTTA CCAACACTTATTCTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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