Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 09/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collected in a field of tall grass using a sweep net. |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | It is very clear that there is no wolbachia present in the dna of my insect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
DEM-JDH
DEM-KMC Lepidoptera
DEM-CJH Blattodea
DEMASCV – Dermaptera
GRE-AMG Diptera