DEMSMC

November 3, 2026

Sample information
Picture
Entry by: Savannah C
Location
Approximate location: 31.97, -81.16
Collection date 08/25/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected in the soil around the base of a large tree
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel electrophoresis yielded no band to indicate the presence of Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
GATTAGGACAGCCNGG NNNNNNNNNTNNNNANNATCANATTTATAATGNTATTGTTACCGNTCANGCTTTTATTATAATTTTTTTTATANTTATGC CTATTATANTCGGGGGATTTGGGAATTGATTANTCCCCTTAATATTAAGAGCCCCCGANATGGCTTTCCCNNNGATAAAT AANATAAAATTTTGATTACTCCCCCCCNCNTTAATGTTATTATTAATGGNANGAATANTANATGCAGGTGCAGGAACCGG NNGAACAGTGNANCCCCNNNTCTCTTCNNTANNTGCTCNCGGANNNNCTNCNNNANATTTGACTATTTTTTCTTTACNCT TGGNGGGGATTTCNNCNNTTTTAGGGGCCATTAATTTTATTACCACGGNGATAAACATACGACCTGAGGGGTTAAAGTTA NAACGAGTTCCTTTATTTGTTNGATCGNNGGCAATTACGGCCCTACTCTTATTAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Dermaptera was found to be negative for Wolbachia.
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