Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 09/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The line of CO1 gene was thin and not as bright, a more above the other lines. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CTCATGNTTTTATCATANTTTTTTTTATAGTTAT ACCTATTATGANNGGAGGATTCGGAAATTGGCTTGTANCTNNNATATNANGCGCCCCTGATATANCNTTCCCTCGAATAA ACAATATAAGATTCTGGCNNNNCCCCCCTTCTCTAACCCTTCTTTTAATAANCAGGCTANTTGAAAATGGAGCCGGAACT GGTTGAACANNNNNNCCCCCNCTGNCAGCTAATATCGCACANAGAGGTGCTTCANTAGATTTANCTATTTTTANCCTTCN TCTAGCAGGAATCNGTTCNNTTCNCGGAGCAGTTAATTTTATTACTACTATAATCAATATACGTTCAATAGGCATAACAT TTGACCGANTANNNTTATTTGCCTGATCAGTTGNACTTACTGCTCTTTTACTTCTCCTATCCCTCCCAGNTNNANCANGA GCTATCNCAATGCTCCTCACTGATCGTAATATC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Diptera was found to be negative for Wolbachia. |
DEM-JDH
DEM-KMC Lepidoptera
DEM-CJH Blattodea
DEMASCV – Dermaptera
GRE-AMG Diptera