DEM-JNH Blattodea

November 3, 2026

Sample information
Picture
Entry by: Jayla Hampton
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

 

  • Time of Collection: 2:14 PM ​
  • Collection method/equipment: Digging in Ground ​
  • Location of Collection: Garden ​
  • Description of Habitat: Grass, Trees, and bushes ​
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

As I looked at the result from doing the gel Electrophoresis system the band that test positive for Wolbachia in the positive control, didn’t in the Blattodea under my result.
Wolbachia 16S sequence
Arthropod COI sequence
TAGGACAACCCGGGTCTCTGATTGGAGACGACCAGATCTACAACGTCATCGTTACCGCCCACGCATTTGTTATAATCTTC TTTATGGTTATACCAATCATAATTGGTGGATTCGGAAACTGACTAGTACCACTTATATTAGGAGCCCCAGATATGGCATT CCCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCATTAACTCTACTACTCACTAGTAGAACTGTAGAAAGTG GAGCAGGGACGGGATGAACAGTTTATCCCCCTCTTGCAAGAGGTATTGCCCATGCAGGAGCATCTGTAGACTTAGCCATC TTCTCCCTACACCTAGCAGGTGTATCATCAATCCTAGGGGCAGTAAACTTTATCTCAACAACAATCAACATAAAAACCAA AAAACATAAAACCCGAACGAATCCCACTATTTGTATGANCTGTCGCCATCACGGCCCTACTCCTACTATTGTCCCTGCCA GTGTTGGCCGGAGCAATCACCATACTACTAACCGACCGCANCCTNNNACATCATTCTTTGATCCAGCCGGANNGGAGATC CNATTCTATACCNACACNTANTCTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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