CCACON Hymenoptera

November 4, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collected in surrounding bushes and foliage of science center
  • Ants found on damp soil covered in fallen leaves and pine needles
  • Other insects found on moist leaves on bushes and trees 
  • Collected with beat stick and sheet
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

extracted genomic DNA ratio was 1.7 indicating an good concentration of extracted DNA. Prevalence of Wolbachia in local dataset in  Hymenoptera only 10%. Positive and Negative controls were accurate
Wolbachia 16S sequence
Arthropod COI sequence
AATAATCATCCGATTAGAACTAGGA TCTTGTAATTCTCTAATTAATAATGATCAAATCTACAACTCCCTAGTTACTAGACACGCTTTTATTATAATTTTTTTTAT AGTTATGCCCTTCATAATTGGGGGATTTGGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCTGATATGGCCTATCCTC GAATAAATAATATAAGATTTTGACTTCTACCACCTTCTCTAACTCTTTTACTTATCAGAAGATTTATTAATAGAGGAGTG GGGACAGGATGAACTATCTATCCACCACTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGACCTATCTATTTTTTC CTTACATATCGCCGGAATATCATCAATCTTAGGGGCTATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATT TTACTATAGATAAAATCCCCCTACTAGTCTGATCTATCCTTATTACAGCCATTCTTCTTTTACTTTCCCTCCCAGTTCTT GCAGGAGCTATTACAATATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGGGAGGGGGAGATCCCAN TCTATATCAACATTTTATTCTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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