Spotted Cucumber Beetle – Diabrotica Undecimpunctata

November 5, 2026

Sample information
Picture
Entry by: Persephone H.
Location
Approximate location: 40.56, -75.40
Collection date
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Coleoptera Chrysomelidae Diabrotica Diabrotica undecimpunctata

Methods
Extraction kit Qiagen DNeasy Blood + Tissue Kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
 DNA Standard
  1. AYPHF1 + AYPHF7
  2. AYPHF2 + AYPHF8
  3. AYPHF3 + AYPHF9
  4. AYPHF4 + AYPHF10
  5. AYPHF5 + AYPHF11
  6. AYPHF6 + AYPHF12
  7. empty
  8. DNA Standard
  9. VALEE1 + VALEE7
  10. VALEE2 + VALEE8
  11. VALEE3 + VALEE9
  12. VALEE4 + VALEE10
  13. VALEE5 + VALEE11
  14. VALEE6 + VALEE12
  15. empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

PCR testing used. Controls worked as expected.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GNTTNNTGATCTGGAATACTAGGATCATCAATGAGACTTATTATTCGAATAGAATTAAGATCCCCAGGATCATGAATTAA CAATGATCAAATTTATAATACAATTGTTACTAGTCATGCATTCCTAATAATTTTTTTTATAGTTATACCATTTTTAATTG GAGGATTTGGAAATTGGCTTATTCCTTTAATACTAGGATCACCTGATATAGCATTCCCCCGAATAAATAATATTAGATTT TGATTACTTCCTCCCTCATTATTTATACTTTTATTAAGAAATTTATTTTATCCAAGACCAGGAACTGGATGAACAGTATA TCCACCATTATCAGCATATTTATATCATTCTTCACCTTCAGTAGATTTTGCAATTTTTTCTCTTCATATATCAGGAATTT CCTCAATTATAGGATCATTAAACTTAATAGTTACAATTATAATAATAAAAAATTTTTCTATAAATTATGACCAAATTTCA TTATTTCCATGATCAGTTTTTATTACAGCAATTTTATTAATTATATCATTACCTGTATTAGCTGGAGCAATTACTATACT ATTATTTGATCGAAATTTTAATACATCATTTTTCGATCCTATAGGAGGTGGAGATCCAATTCTTTATCAACATTTATTTT GATTTNTGNNNNNNNNNGGANNNNNTNTNNNNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diabrotica undecimpunctata was found to be negative for Wolbachia.
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