japanese burrowing cricket

October 6, 2021

Sample information
Picture
Photos by: Mira B.
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

When put into the sanger sequence site, the sequence came out to be 100 percent accurate and 100 percent a match. This shows that the morphology and nucelotide sequencing were effective and successful, as well as accurate by a lot.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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