Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Found on a dead tree, can fly. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Asilidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The electrophoresis PCR showed a very prominent line positive for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Asilidae was found to be postive for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota