Robber Fly

October 6, 2021

Sample information
Picture
Photos by: Nicole
Location
Collection date 08/22/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Found on a dead tree, can fly.
Putative identification Arthropoda Hexapoda Insecta Diptera Asilidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The electrophoresis PCR showed a very prominent line positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Asilidae was found to be postive for Wolbachia.
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