Stink Bug

September 7, 2023

Sample information
Picture
Photos by: Tendai B
Location
Collection date 09/22/2021
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

brown
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: I made sure to crush the arthropod really well. I dissected the abdomen, and did not include the ext

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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