Sample information
Picture	Photos by: Supria S.
Location
Collection date	09/13/2021
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations
Putative identification	Arthropoda Chelicerata Arachnida Araneae Cheiracanthiidae Cheiracanthium Cheiracanthium mildei
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Cheiracanthium mildei was found to be postive for Wolbachia.