Common black ground beetle (Ella Rader)

April 5, 2022

Sample information
Picture
Photos by: Ella Rader
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Black beetle, small with lines on the back.
Putative identification Arthropoda Insecta Coleoptera Carabidae Pterostichus

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

My DNA for my bug was the only one that showed on the gel. My bug was negative for Wolbachia.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I know that the common black ground beetle i found was negative for Wolbachia because i got the DNA but the wolbachia slot was negative. I’m confident because we followed the protocol exactly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pterostichus was found to be negative for Wolbachia.
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