Centipede

April 18, 2022

Sample information
Picture
Photos by: Mallory C
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Yellow centipede, small, found under a rock with wet dirt, and a lot of legs, “scaly”, little antennas
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

Confidence level would be medium because we think the testing got messed up and the positives and negative control did not show up in the gel when we put the black light on it
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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