Solider Ant

April 27, 2022

Sample information
Picture
Photos by: Sara K.
Location
Collection date 04/11/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

– moves quicker than ants around them, found in damp soil.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

– confidence is low due to the fact that the positive control was not an arthropod and the negative control had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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