Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/07/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Red-ish-brown, six legs, an inch long, antennae an inch and a half, two sets of wings, external shell, hair plates |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Either there was contamination in the gel, or the DNA was placed incorrectly into the gel. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Both the negative control and the positive control had Wolbachia and the positive did not show arthropod DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Blattodea was found to be negative for Wolbachia. |
Water Boatman