CARPENTER ANT ( SYNIYA HARRIS)

Sample information

Picture
Location
Collection date 04/12/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • my arthropod was near leaves and trees kinda close to sap I found on a tree. Because it is an ant it was found with a cluster of others. To allow for easier catching I put an apple on my deck to allow for a few to come. To my surprise  many an f I was able to collect. The ant was crawling  around taking some of the apple pieces back to the tree. Some descriptions of my ant would be it’s size and color. This carpenter ant was a burgundy color and quite small in size. Compared to the other ants crawling around it the size was quite small but the burgundy stood out. It had hints of black ( the antenna and other small features).
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Honestly, they weren’t any mistakes or difficulties. The only one I can point out is the fact that our pipettes were breaking, and also we couldn’t complete the last part of our experiment. However, it was completed by another class. Although there were only a few hiccups throughout the way a few mistakes were made. Whether that be not understanding what to do or not paying, much attention.

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Because I wasn’t able to completely observe my data and also feel as If I know enough information to form I feel medium about my data. I do believe the data is correct however I think that there might have been a misreading. Although I do feel this way I think my data was clear and precise.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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