Sample information |
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Picture |
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Location | |
Collection date | 04/12/2022 |
Captive / Cultivated? | Wild-caught |
Group | Hampton Roads Academy |
Observations |
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Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
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Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | Honestly, they weren’t any mistakes or difficulties. The only one I can point out is the fact that our pipettes were breaking, and also we couldn’t complete the last part of our experiment. However, it was completed by another class. Although there were only a few hiccups throughout the way a few mistakes were made. Whether that be not understanding what to do or not paying, much attention. |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | Because I wasn’t able to completely observe my data and also feel as If I know enough information to form I feel medium about my data. I do believe the data is correct however I think that there might have been a misreading. Although I do feel this way I think my data was clear and precise. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Hymenoptera was found to be negative for Wolbachia. |