Orange Headed Epicallima Moth

April 26, 2022

Sample information
Picture
Location
Collection date 09/15/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

• 2 winged
• 2 antennae
• 2 black-eyes (located in front right below antennae)
• Tan/beige color
• Rolled up sucker mouth (Like a butterfly)
• 3 sets of legs
• Legs have hair/is jagged
• 3 segments
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Potential Mistakes:

  • DNA extraction wasn’t successful (didn’t extract enough)
  • Didn’t place DNA solution correctly inside the cell of the gel
  • Something wrong with PCR Master Mix

Pellet Description:

  • Small, dark brown, rested at bottom of microcentrifuge tube

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level
  • Didn’t get arthropod band
  • Positive control didn’t show bands (results might not be accurate)

 
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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