Lasius sp. IA

May 10, 2022

Sample information
Picture
Photos by: Ilaria Turgut
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found the ant under the bark of a tree. I lifted it with a pincette.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

i made sure to crush the arthropod really well, but include the exoskeleton. the part from the arthropod that i mortared was from head to abdomen.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

it clearly has only one band and not two
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius was found to be negative for Wolbachia.
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