Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/29/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | i found the arthropod under the bark of a tree |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I made sure to crush the arthropod really well. There was only one band, there could have been a problem with my DNA extraction. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | because the results show that there is only one band and not two |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman