Beetle IK

May 10, 2022

Sample information
Picture
Photos by: Ilaria Turgut
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

i found the arthropod under the bark of a tree
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

I made sure to crush the arthropod really well. There was only one band, there could have been a problem with my DNA extraction.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

because the results show that there is only one band and not two
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post