Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida Julidae Tachypodoiulus Tachypodoiulus niger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We cut the organism lengthwise in 4 parts from head to end and take the third quarter |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | No bands of amplification are visible Line 02: no CO1-Band, no W-Band in the same series – Drosophila positive control Vanderbilt worked well, see BK26 |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
DKH10
DKH 9
DKH8
DKH 7
DKH 6